| 质粒名称: | 5aa SunTag VP64 CLV3 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 115485 |
| 存储实验室: | Steven Jacobsen |
|
载体骨架基本信息 |
|
| 载体名称: | pMOA34 |
| 载体出品公司: | -- |
| 载体抗性: | 大观霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 植物表达，CRISPR，合成生物学 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS,5aa SunTag VP64 CLV3 |
| 物种来源: | -- |
| 插入大小: | 15407 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | 5aa SunTag VP64 CLV3 was a gift from Steven Jacobsen (Addgene plasmid # 115485 ; http://n2t.net/addgene:115485 ; RRID:Addgene_115485) |
| 参考文献部分: | Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems. Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE. Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7. 10.1038/s41467-019-08736-7 PubMed 30760722 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS 5aa SunTag VP64 CLV3 26054 bp ds-DNA circular SYN 21-MAR-2019
DEFINITION CRISPR-Cas9 SunTag system to target VP64 to the CLV3 locus with two
guide RNAs.
ACCESSION .
VERSION .
KEYWORDS 5aa SunTag VP64 CLV3
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 26054)
AUTHORS Papikian A, Liu W, Gallego-Bartolome J, Jacobsen SE
TITLE Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9
SunTag systems.
JOURNAL Nat Commun. 2019 Feb 13;10(1):729. doi: 10.1038/s41467-019-08736-7.
PUBMED 30760722
REFERENCE 2 (bases 1 to 26054)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 21, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..26054
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 4614..4638
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
misc_feature 4657..4712
/label=TMV Omega
/note="translational enhancer from the tobacco mosaic virus
5'-leader sequence (Gallie et al., 1988)"
CDS 4726..8829
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8833..8859
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 8878..8898
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8905..8925
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 8989..9045
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9061..9117
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9133..9189
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9205..9261
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9277..9333
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9349..9405
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9421..9477
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9493..9549
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9565..9621
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
CDS 9637..9693
/codon_start=1
/product="GCN4 peptide optimized to improve solubility
while preserving binding to an scFv-GFP fusion protein
(Tanenbaum et al., 2014)"
/label=GCN4_v4
/translation="EELLSKNYHLENEVARLKK"
terminator 9741..10448
/label=OCS terminator
/note="octopine synthase terminator"
promoter complement(10481..10499)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(10482..10499)
/label=SP6
/note="SP6 promoter, forward primer"
misc_feature 10542..10566
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
oriT 11128..11237
/note="incP origin of transfer"
mobile_element 11297..12064
/mobile_element_type="insertion sequence:IS1"
/note="prokaryotic transposable element"
CDS 12689..13837
/codon_start=1
/product="trans-acting replication protein that binds to
and activates oriV"
/label=trfA
/translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
primer_bind complement(15026..15043)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(15197..15785)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(15277..15296)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS 16882..17670
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 18345..18876
/label=oriV
/note="incP origin of replication"
rep_origin 18353..18967
/label=oriV
/note="origin of replication for the bacterial F plasmid"
misc_feature 19067..19091
/label=LB T-DNA repeat
/note="left border repeat from octopine Ach5 T-DNA"
terminator 19165..19417
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(19445..20470)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
E"
promoter complement(20524..20703)
/label=NOS promoter
/note="nopaline synthase promoter"
misc_RNA complement(21161..21236)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_RNA complement(21718..21793)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
CDS complement(22551..22601)
/codon_start=1
/product="nuclear localization signal from the human T-cell
leukemia virus type 1 (HTLV-1) Rex protein"
/label=Rex NLS
/translation="PKTRRRPRRSQRKRPPT"
CDS complement(22629..22790)
/codon_start=1
/product="B1 domain of Streptococcal protein G "
/label=GB1
/note="effective as a solubilizing fusion partner (Cheng
and Patel, 2004)"
/translation="YKLILNGKTLKGETTTEAVDAATAEKVFKQYANDNGVDGEWTYDD
ATKTFTVTE"
CDS complement(22821..22841)
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(22866..23015)
/codon_start=1
/product="tetrameric repeat of the minimal activation
domain of herpes simplex virus VP16 (Beerli et al., 1998)"
/label=VP64
/translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
DLDML"
CDS complement(23055..23765)
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
primer_bind complement(23095..23115)
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
primer_bind 23710..23738
/label=GFP-R
/note="GFP, reverse primer. Does NOT anneal to EGFP"
CDS complement(23856..23882)
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
ORIGIN
1 agaagatgca gatgagtgga ttagaaggaa agaggctcag aatgtaagga aagcaccaac
61 cacaacaccg tcgggacaga cgagaaacat gggaagctcc accacaatct tgggttaagt
121 gcaactttga tggggcatgg ccaacagaag gattaaaatg tggcttaggg tgggtgcttc
181 gcgatcatac agggaaggtg ttatggttag gtgcaagagc tgtggtaaaa gtaagaagcg
241 tgctggaagt agaagtggag gctcttagat gggctgtgct gtcattatcc cgattcaatt
301 ataggaagat catttttgag gtggattctc agcaacttgt gtctttggtt acatgaaagt
361 tatgcttgtc aagtctcaat ccaattatcc aagacataaa gtatctactt agcaagtttg
421 aggattttat gcttgtgcat acaagccgag aaggaaatgg agtggcagat agaatagcta
481 aggaatctct ttcttttgag aattatgatc taaagttgta ttctattgta ccaatttggg
541 ttaaaagctc tgttgagcta gactcatatg catcctagga aacaagttgt aatgagttgc
601 tggcctctct tgggattgtt catcaaagta gctgtcccta cacaccacaa cagaatggtg
661 ttgtggagag aaagcacaga cacatccttg agatggcaag ggcacttaag tttcagagtg
721 gtgtacctac caggttttgg ggagactgtg tcaagactgt tgtatacctg atcaatagac
781 tccctactcc aatcttacaa ggcaaatgtc catatgaact actctatcag aaacatgcca
841 agcttgatca cttgagagtg tttgggtgtc ttgcttttgc agcaacactg cccaaaggtg
901 acaaacttgc acctagagcc aagcgaacca tcttcattgg ttattctgag actcaaaagg
961 gttacaggtt gtatgacttg gataataagg tgatcatagt gagcagggat gtagttttca
1021 gagagtttca gttccctttc aaagagtcca tctcccatga acctgatatg ttcactcgag
1081 caggttcact tagctctgaa gatacagctt tgcagctagt gtataatgat atttttcctt
1141 ccagctttca tactgatgat gatgatactc cagttcttat tcctgacatt gtagaggcca
1201 atgaggatat cactttgggg actcattcta cccaaaatca tatcacttca gctgatgctg
1261 caccttcagg ccacctgcat gctcctgcag agtcagttga gtcagctgag cctgacctag
1321 caaatgctga atttgagcac catactgctg tagctgatcc ctctctagtt ccagcaaacc
1381 tgcacactag acctaaacgc aatgcaggtc ctcccatctg gctcaaggac tttgtgacac
1441 tgaacaaagg ctctagggat gttccatacc ctatagccaa ctatgtttcc tatgatcact
1501 tgcctgttca ctatcaagct tatttgagtg ctttctccac ttatactgaa cctaaatcct
1561 tcaaagaagc agctcaagat gagaaatgga tggaggccat gtcccttgag atacaggctc
1621 ttgaggataa taacacctgg gagattgtcc ctttaccccc tggtaaacag cctatagggt
1681 ccaaatgggt gtacaaaatt aaatacaaag ctaatggtga agttgacagg tttaaggcaa
1741 ggctagtggc caagggatac actcagcaag aaggccttga ctaccatgaa actttttctc
1801 cagtggccaa aatggtcact gtaaaagctg tcatatctgt tgctgcttcc aagggctggt
1861 tccttttcca gatggatgtc aacaatgctt ttctacaagg tgacctcatg gaagaagttt
1921 atatgtctct gcctcagggt tttcacagcc aaggggagac caaagtgtgc aggctgttga
1981 atccctctat ggtttaaagc aagcatcaag gcagtggaac atcaagctta ccactgtcct
2041 tatgcaggct ggttttatgc aaagtgctta tgatcactcc ttgtttacca aaagagaggg
2101 gactgacctt gtcataatcc tgatttatgt ggatgatctg ttaataacag gcagcagcaa
2161 tgttttgatt tcagaagcaa aggcaaccct gcatcagcat ttcaagatga aagatttggg
2221 agaactaaaa tacttcttag gcattgaggt gctgagatca gaaaagggaa tcctactgaa
2281 ccagaggaag tatgcactgg aactgatatc aggtgttggt ctgggaggct gcagaccagt
2341 gtcaacccca atggaacaga accaaaggct gactactgta gaatatgaca agcacttagg
2401 aaagactgat gatgcagagt tagaggatgt gggttcctat cagagactgg tgggaaagct
2461 tctctacttg acaatcacaa ggccagatat cagctttgca gtgcaggtgc tgagtcaatt
2521 catgcagcaa cccaaacagt cacacctgga agcagcattg agggttgtga agtacataaa
2581 aggttctcca ggtgttagga agcggccgca tgcatatgag tctagctcaa cagagctttt
2641 aacccaaatt ggtacaatag aatacaactt tagatcataa ttctcaaaag aaagagattc
2701 cttagctatt ctatctgcca ctccatttcc ttctcggctt gtatgcacaa gcataaaatc
2761 ctcaaacttg ctaagtagat actttatgtc ttggataatt ggattgagac ttgacaagca
2821 taactttcat gtaaccaaag acacaagttg ctgagaatcc acctcaaaaa tgatcttcct
2881 ataattgaat cgggataatg acagcacagc ccatctaaga gcctccactt ctacttccag
2941 cacgcttctt acttttacca cagctcttgc acctaaccat aacaccttcc ctgtatgatc
3001 gcgaagcacc caccctaagc cacattttaa tccttctgtt ggccatgccc catcaaagtt
3061 gcacttaacc caagattgtg gtggagcttc ccatgtttct cgtctgtccc gacggtgttg
3121 tggttggtgc tttccttaca ttctgagcct ctttccttct aatccactca tctgcatctt
3181 cttgtgtcct tactaatacc tcattggttc caaattccct ccctttaagc accagctcgt
3241 ttctgttctt ccacagcctc ccaagtatcc aagggactaa agcctccaca ttcttcagat
3301 caggatattc ttgtttaaga tgttgaactc tatggaggtt tgtatgaact gatgatctag
3361 gaccggataa gttcccttct tcatagcgaa cttattcaaa gaatgttttg tgtatcattc
3421 ttgttacatt gttattaatg aaaaaatatt attggtcatt ggactgaaca cgagtgttaa
3481 atatggacca ggccccaaat aagatccatt gatatatgaa ttaaataaca agaataaatc
3541 gagtcaccaa accacttgcc ttttttaacg agacttgttc accaacttga tacaaaagtc
3601 attatcctat gcaaatcaat aatcatacaa aaatatccaa taacactaaa aaattaaaag
3661 aaatggataa tttcacaata tgttatacga taaagaagtt acttttccaa gaaattcact
3721 gattttataa gcccacttgc attagataaa tggcaaaaaa aaacaaaaag gaaaagaaat
3781 aaagcacgaa gaattctaga aaatacgaaa tacgcttcaa tgcagtggga cccacggttc
3841 aattattgcc aattttcagc tccaccgtat atttaaaaaa taaaacgata atgctaaaaa
3901 aatataaatc gtaacgatcg ttaaatctca acggctggat cttatgacga ccgttagaaa
3961 ttgtggttgt cgacgagtca gtaataaacg gcgtcaaagt ggttgcagcc ggcacacacg
4021 agtcgtgttt atcaactcaa agcacaaata cttttcctca acctaaaaat aaggcaatta
4081 gccaaaaaca actttgcgtg taaacaacgc tcaatacacg tgtcatttta ttattagcta
4141 ttgcttcacc gccttagctt tctcgtgacc tagtcgtcct cgtcttttct tcttcttctt
4201 ctataaaaca atacccaaag agctcttctt cttcacaatt cagatttcaa tttctcaaaa
4261 tcttaaaaac tttctctcaa ttctctctac cgtgatcaag gtaaatttct gtgttcctta
4321 ttctctcaaa atcttcgatt ttgttttcgt tcgatcccaa tttcgtatat gttctttggt
4381 ttagattctg ttaatcttag atcgaagacg attttctggg tttgatcgtt agatatcatc
4441 ttaattctcg attagggttt catagatatc atccgatttg ttcaaataat ttgagttttg
4501 tcgaataatt actcttcgat ttgtgatttc tatctagatc tggtgttagt ttctagtttg
4561 tgcgatcgaa tttgtcgatt aatctgagtt tttctgatta acagggatca tcaacaagtt
4621 tgtacaaaaa agcaggctct ttaaagtatt tttacaacaa ttaccaacaa caacaaacaa
4681 caaacaacat tacaattact atttacaatt acaaaaaaag ttaacatgga caagaagtac
4741 agcatcggcc tggccatcgg caccaactct gtgggctggg ccgtgatcac cgacgagtac
4801 aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg accggcacag catcaagaag
4861 aacctgatcg gcgccctgct gttcgacagc ggagaaacag ccgaggccac ccggctgaag
4921 agaaccgcca gaagaagata caccagacgg aagaaccgga tctgctatct gcaagagatc
4981 ttcagcaacg agatggccaa ggtggacgac agcttcttcc acagactgga agagtccttc
5041 ctggtggaag aggataagaa gcacgagcgg caccccatct tcggcaacat cgtggacgag
5101 gtggcctacc acgagaagta ccccaccatc taccacctga gaaagaaact ggtggacagc
5161 accgacaagg ccgacctgcg gctgatctat ctggccctgg cccacatgat caagttccgg
5221 ggccacttcc tgatcgaggg cgacctgaac cccgacaaca gcgacgtgga caagctgttc
5281 atccagctgg tgcagaccta caaccagctg ttcgaggaaa accccatcaa cgccagcggc
5341 gtggacgcca aggccatcct gtctgccaga ctgagcaaga gcagacggct ggaaaatctg
5401 atcgcccagc tgcccggcga gaagaagaat ggcctgttcg gcaacctgat tgccctgagc
5461 ctgggcctga cccccaactt caagagcaac ttcgacctgg ccgaggatgc caaactgcag
5521 ctgagcaagg acacctacga cgacgacctg gacaacctgc tggcccagat cggcgaccag
5581 tacgccgacc tgtttctggc cgccaagaac ctgtccgacg ccatcctgct gagcgacatc
5641 ctgagagtga acaccgagat caccaaggcc cccctgagcg cctctatgat caagagatac
5701 gacgagcacc accaggacct gaccctgctg aaagctctcg tgcggcagca gctgcctgag
5761 aagtacaaag agattttctt cgaccagagc aagaacggct acgccggcta catcgatggc
5821 ggagccagcc aggaagagtt ctacaagttc atcaagccca tcctggaaaa gatggacggc
5881 accgaggaac tgctcgtgaa gctgaacaga gaggacctgc tgcggaagca gcggaccttc
5941 gacaacggca gcatccccca ccagatccac ctgggagagc tgcacgccat tctgcggcgg
6001 caggaagatt tttacccatt cctgaaggac aaccgggaaa agatcgagaa gatcctgacc
6061 ttccgcatcc cctactacgt gggccctctg gccaggggaa acagcagatt cgcctggatg
6121 accagaaaga gcgaggaaac catcaccccc tggaacttcg aggaagtggt ggacaagggc
6181 gccagcgccc agagcttcat cgagcggatg accaacttcg ataagaacct gcccaacgag
6241 aaggtgctgc ccaagcacag cctgctgtac gagtacttca ccgtgtacaa cgagctgacc
6301 aaagtgaaat acgtgaccga gggaatgaga aagcccgcct tcctgagcgg cgagcagaaa
6361 aaagccatcg tggacctgct gttcaagacc aaccggaaag tgaccgtgaa gcagctgaaa
6421 gaggactact tcaagaaaat cgagtgcttc gactccgtgg aaatctccgg cgtggaagat
6481 cggttcaacg cctccctggg cacataccac gatctgctga aaattatcaa ggacaaggac
6541 ttcctggaca atgaggaaaa cgaggacatt ctggaagata tcgtgctgac cctgacactg
6601 tttgaggaca gagagatgat cgaggaacgg ctgaaaacct atgcccacct gttcgacgac
6661 aaagtgatga agcagctgaa gcggcggaga tacaccggct ggggcaggct gagccggaag
6721 ctgatcaacg gcatccggga caagcagtcc ggcaagacaa tcctggattt cctgaagtcc
6781 gacggcttcg ccaacagaaa cttcatgcag ctgatccacg acgacagcct gacctttaaa
6841 gaggacatcc agaaagccca ggtgtccggc cagggcgata gcctgcacga gcacattgcc
6901 aatctggccg gcagccccgc cattaagaag ggcatcctgc agacagtgaa ggtggtggac
6961 gagctcgtga aagtgatggg ccggcacaag cccgagaaca tcgtgatcga aatggccaga
7021 gagaaccaga ccacccagaa gggacagaag aacagccgcg agagaatgaa gcggatcgaa
7081 gagggcatca aagagctggg cagccagatc ctgaaagaac accccgtgga aaacacccag
7141 ctgcagaacg agaagctgta cctgtactac ctgcagaatg ggcgggatat gtacgtggac
7201 caggaactgg acatcaaccg gctgtccgac tacgatgtgg acgctatcgt gcctcagagc
7261 tttctgaagg acgactccat cgataacaaa gtgctgactc ggagcgacaa gaaccggggc
7321 aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga tgaagaacta ctggcgccag
7381 ctgctgaatg ccaagctgat tacccagagg aagttcgaca atctgaccaa ggccgagaga
7441 ggcggcctga gcgaactgga taaggccggc ttcatcaaga gacagctggt ggaaacccgg
7501 cagatcacaa agcacgtggc acagatcctg gactcccgga tgaacactaa gtacgacgag
7561 aacgacaaac tgatccggga agtgaaagtg atcaccctga agtccaagct ggtgtccgat
7621 ttccggaagg atttccagtt ttacaaagtg cgcgagatca acaactacca ccacgcccac
7681 gacgcctacc tgaacgccgt cgtgggaacc gccctgatca aaaagtaccc taagctggaa
7741 agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc ggaagatgat cgccaagagc
7801 gagcaggaaa tcggcaaggc taccgccaag tacttcttct acagcaacat catgaacttt
7861 ttcaagaccg agattaccct ggccaacggc gagatccgga agcggcctct gatcgagaca
7921 aacggcgaaa caggcgagat cgtgtgggat aagggccggg actttgccac cgtgcggaaa
7981 gtgctgtcta tgccccaagt gaatatcgtg aaaaagaccg aggtgcagac aggcggcttc
8041 agcaaagagt ctatcctgcc caagaggaac agcgacaagc tgatcgccag aaagaaggac
8101 tgggacccta agaagtacgg cggcttcgac agccccaccg tggcctattc tgtgctggtg
8161 gtggccaaag tggaaaaggg caagtccaag aaactgaaga gtgtgaaaga gctgctgggg
8221 atcaccatca tggaaagaag cagcttcgag aagaatccca tcgactttct ggaagccaag
8281 ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc ctaagtactc cctgttcgag
8341 ctggaaaacg gccggaagag aatgctggcc tctgccggcg aactgcagaa gggaaacgaa
8401 ctggccctgc cctccaaata tgtgaacttc ctgtacctgg ccagccacta tgagaagctg
8461 aagggctccc ccgaggataa tgagcagaaa cagctgtttg tggaacagca caaacactac
8521 ctggacgaga tcatcgagca gatcagcgag ttctccaaga gagtgatcct ggccgacgct
8581 aatctggaca aggtgctgag cgcctacaac aagcacagag acaagcctat cagagagcag
8641 gccgagaata tcatccacct gtttaccctg accaatctgg gagcccctgc cgccttcaag
8701 tactttgaca ccaccatcga ccggaagagg tacaccagca ccaaagaggt gctggacgcc
8761 accctgatcc accagagcat caccggcctg tacgagacac ggatcgacct gtctcagctg
8821 ggaggcgacg cctatcccta tgacgtgccc gattatgcca gcctgggcag cggctccccc
8881 aagaaaaaac gcaaggtgga agatcctaag aaaaagcgga aagtggacgg cattggtagt
8941 gggagcaacg gcagcagcgg atccaacggt ccgactgacg ccgcggaaga agaacttttg
9001 agcaagaatt atcatcttga gaacgaagtg gctcgtctta agaaaggttc tggcagtgga
9061 gaagaactgc tttcaaagaa ttaccacctg gaaaatgagg tagctagact gaaaaagggg
9121 agcggaagtg gggaggagtt gctgagcaaa aattatcatt tggagaacga agtagcacga
9181 ctaaagaaag ggtccggatc gggtgaggag ttactctcga aaaattatca tctcgaaaac
9241 gaagtggctc ggctaaaaaa gggcagtggt tctggagaag agctattatc taaaaactac
9301 cacctcgaaa atgaggtggc acgcttaaaa aagggaagtg gcagtggtga agagctacta
9361 tccaagaatt atcatcttga gaacgaggta gcgcgtttga agaagggttc cggctcagga
9421 gaggaactgc tctcgaagaa ctatcatctt gaaaatgagg tcgctcgatt aaaaaaggga
9481 tcgggcagtg gtgaggaact actttcaaag aattaccacc tcgaaaacga agtagctcga
9541 ttaaagaaag gttcagggtc gggtgaagaa ttactgagta aaaattatca tctggaaaat
9601 gaggtagcga gactaaaaaa ggggagtggt tctggcgagg aattgctatc gaaaaattat
9661 catcttgaga acgaagttgc taggctcaaa aagggctcag gctcaggcta acccgggggc
9721 gcgccatgat aactagagtc ctgctttaat gagatatgcg agacgcctat gatcgcatga
9781 tatttgcttt caattctgtt gtgcacgttg taaaaaacct gagcatgtgt agctcagatc
9841 cttaccgccg gtttcggttc attctaatga atatatcacc cgttactatc gtatttttat
9901 gaataatatt ctccgttcaa tttactgatt gtaccctact acttatatgt acaatattaa
9961 aatgaaaaca atatattgtg ctgaataggt ttatagcgac atctatgata gagcgccaca
10021 ataacaaaca attgcgtttt attattacaa atccaatttt aaaaaaagcg gcagaaccgg
10081 tcaaacctaa aagactgatt acataaatct tattcaaatt tcaaaagtgc cccaggggct
10141 agtatctacg acacaccgag cggcgaacta ataacgctca ctgaagggaa ctccggttcc
10201 ccgccggcgc gcatgggtga gattccttga agttgagtat tggccgtccg ctctaccgaa
10261 agttacgggc accattcaac ccggtccagc acggcggccg ggtaaccgac ttgctgcccc
10321 gagaattatg cagcattttt ttggtgtatg tgggccccaa atgaagtgca ggtcaaacct
10381 tgacagtgac gacaaatcgt tgggcgggtc cagggcgaat tttgcgacaa catgtcgagg
10441 ctcagcagga ccggcatgca agctagctta ctagtgatat tctatagtgt cacctaaatc
10501 tgcggccgcg gtaccactag tacgcgtagg cctgagctct ttgacaggat atattggcgg
10561 gtaaacgata tcggcacctt cgaccgcctg ttccgcagag gtgcaggcct cgatctgaaa
10621 cccgaaccgc tggagattgc gggagcagcg agcagtagcc tcggggtcga tgtcgtaaag
10681 tcgtatccga tcgacgccga tcagcgcctt gaaggccaaa gcctggaact cactttgggc
10741 accgttgccg atcagcgcca tcgtgcgcga atctttacgg gccagatact ttgccgcgat
10801 cgcggaggtc gcggccgttc gcaaggccgt caggattgtc atttccgaca gcagcagcgg
10861 atagccgcta tcgacatcgg agagcacgcc gaacgcggtt accggcctct tcatcgggaa
10921 tgcgcgcgac cttcagcatc gccggcatgt ccccctggcg gacgggaagt atccagctcg
10981 acctcgacgg atcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc
11041 ggtatatcca tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt
11101 tccttggtgt atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag
11161 cgggtgttcc ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc
11221 tctgcgaggc tggccggcta ccgccggcgt aacagatgag ggcaagcgga tggctgatga
11281 aaccaagcca accaggggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt
11341 gaggtgctcc agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg
11401 tggtgcgtaa cggcaaaagc accgccggac atcagcgcta tctctgctct cactgccgta
11461 aaacatggca actgcagttc acttacaccg cttctcaacc cggtacgcac cagaaaatca
11521 ttgatatggc catgaatggc gttggatgcc gggcaacagc ccgcattatg ggcgttggcc
11581 tcaacacgat tttacgtcac ttaaaaaact caggccgcag tcggtaacct cgcgcataca
11641 gccgggcagt gacgtcatcg tctgcgcgga aatggacgaa cagtggggct atgtcggggc
11701 taaatcgcgc cagcgctggc tgttttacgc gtatgacagt ctccggaaga cggttgttgc
11761 gcacgtattc ggtgaacgca ctatggcgac gctggggcgt cttatgagcc tgctgtcacc
11821 ctttgacgtg gtgatatgga tgacggatgg ctggccgctg tatgaatccc gcctgaaggg
11881 aaagctgcac gtaatcagca agcgatatac gcagcgaatt gagcggcata acctgaatct
11941 gaggcagcac ctggcacggc tgggacggaa gtcgctgtcg ttctcaaaat cggtggagct
12001 gcatgacaaa gtcatcgggc attatctgaa cataaaacac tatcaataag ttggagtcat
12061 tacccaacca ggaagggcag cccacctatc aaggtgtact gccttccaga cgaacgaaga
12121 gcgattgagg aaaaggcggc ggcggccggc atgagcctgt cggcctacct gctggccgtc
12181 ggccagggct acaaaatcac gggcgtcgtg gactatgagc acgtccgcga gctggcccgc
12241 atcaatggcg acctgggccg cctgggcggc ctgctgaaac tctggctcac cgacgacccg
12301 cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc tggcgaagat cgaagagaag
12361 caggacgagc ttggcaaggt catgatgggc gtggtccgcc cgagggcaga gccatgactt
12421 ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc aagcacgtcc ccatgcgctc
12481 catcaagaag agcgacttcg cggagctggt attcgtgcag ggcaagattc ggaataccaa
12541 gtacgagaag gacggccaga cggtctacgg gaccgacttc attgccgata aggtggatta
12601 tctggacacc aaggcaccag gcgggtcaaa tcaggaataa gggcacattg ccccggcgtg
12661 agtcggggca atcccgcaag gagggtgaat gaatcggacg tttgaccgga aggcatacag
12721 gcaagaactg atcgacgcgg ggttttccgc cgaggatgcc gaaaccatcg caagccgcac
12781 cgtcatgcgt gcgccccgcg aaaccttcca gtccgtcggc tcgatggtcc agcaagctac
12841 ggccaagatc gagcgcgaca gcgtgcaact ggctccccct gccctgcccg cgccatcggc
12901 cgccgtggag cgttcgcgtc gtctcgaaca ggaggcggca ggtttggcga agtcgatgac
12961 catcgacacg cgaggaacta tgacgaccaa gaagcgaaaa accgccggcg aggacctggc
13021 aaaacaggtc agcgaggcca agcaggccgc gttgctgaaa cacacgaagc agcagatcaa
13081 ggaaatgcag ctttccttgt tcgatattgc gccgtggccg gacacgatgc gagcgatgcc
13141 aaacgacacg gcccgctctg ccctgttcac cacgcgcaac aagaaaatcc cgcgcgaggc
13201 gctgcaaaac aaggtcattt tccacgtcaa caaggacgtg aagatcacct acaccggcgt
13261 cgagctgcgg gccgacgatg acgaactggt gtggcagcag gtgttggagt acgcgaagcg
13321 cacccctatc ggcgagccga tcaccttcac gttctacgag ctttgccagg acctgggctg
13381 gtcgatcaat ggccggtatt acacgaaggc cgaggaatgc ctgtcgcgcc tacaggcgac
13441 ggcgatgggc ttcacgtccg accgcgttgg gcacctggaa tcggtgtcgc tgctgcaccg
13501 cttccgcgtc ctggaccgtg gcaagaaaac gtcccgttgc caggtcctga tcgacgagga
13561 aatcgtcgtg ctgtttgctg gcgaccacta cacgaaattc atatgggaga agtaccgcaa
13621 gctgtcgccg acggcccgac ggatgttcga ctatttcagc tcgcaccggg agccgtaccc
13681 gctcaagctg gaaaccttcc gcctcatgtg cggatcggat tccacccgcg tgaagaagtg
13741 gcgcgagcag gtcggcgaag cctgcgaaga gttgcgaggc agcggcctgg tggaacacgc
13801 ctgggtcaat gatgacctgg tgcattgcaa acgctagggc cttgtggggt cagttccggc
13861 tgggggttca gcagccagcg ctttactggc atttcaggaa caagcgggca ctgctcgacg
13921 cacttgcttc gctcagtatc gctcgggacg cacggcgcgc tctacgaact gccgataaac
13981 agaggattaa aattgacaat tgtgattaag gctcagattc gacggcttgg agcggccgac
14041 gtgcaggatt tccgcgagat ccgattgtcg gccctgaaga aagctccaga gatgttcggg
14101 tccgtttacg agcacgagga gaaaaagccc atggaggcgt tcgctgaacg gttgcgagat
14161 gccgtggcat tcggcgccta catcgacggc gagatcattg ggctgtcggt cttcaaacag
14221 gaggacggcc ccaaggacgc tcacaaggcg catctgtccg gcgttttcgt ggagcccgaa
14281 cagcgaggcc gaggggtcgc cggtatgctg ctgcgggcgt tgccggcggg tttattgctc
14341 gtgatgatcg tccgacagat tccaacggga atctggtgga tgcgcatctt catcctcggc
14401 gcacttaata tttcgctatt ctggagcttg ttgtttattt cggtctaccg cctgccgggc
14461 ggggtcgcgg cgacggtagg cgctgtgcag ccgctgatgg tcgtgttcat ctctgccgct
14521 ctgctaggta gcccgatacg attgatggcg gtcctggggg ctatttgcgg aactgcgggc
14581 gtggcgctgt tggtgttgac accaaacgca gcgctagatc ctgtcggcgt cgcagcgggc
14641 ctggcggggg cggtttccat ggcgttcgga accgtgctga cccgcaagtg gcaacctccc
14701 gtgcctctgc tcacctttac cgcctggcaa ctggcggccg gaggacttct gctcgttcca
14761 gtagctttag tgtttgatcc gccaatcccg atgcctacag gaaccaatgt tctcggcctg
14821 gcgtggctcg gcctgatcgg agcgggttta acctacttcc tttggttccg ggggatctcg
14881 cgactcgaac ctacagttgt ttccttactg ggctttctca gccgggatgg cgctaagaag
14941 ctattgccgc cgatcttcat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
15001 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
15061 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
15121 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
15181 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
15241 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
15301 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
15361 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
15421 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
15481 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
15541 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
15601 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
15661 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
15721 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
15781 atcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
15841 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
15901 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
15961 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
16021 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
16081 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
16141 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
16201 ttaaacaagt ggcagcaacg gattcgcaaa cctgtcacgc cttttgtgcc aaaagccgcg
16261 ccaggtttgc gatccgctgt gccaggcgtt aggcgtcata tgaagatttc ggtgatccct
16321 gagcaggtgg cggaaacatt ggatgctgag aaccatttca ttgttcgtga agtgttcgat
16381 gtgcacctat ccgaccaagg ctttgaacta tctaccagaa gtgtgagccc ctaccggaag
16441 gattacatct cggatgatga ctctgatgaa gactctgctt gctatggcgc attcatcgac
16501 caagagcttg tcgggaagat tgaactcaac tcaacatgga acgatctagc ctctatcgaa
16561 cacattgttg tgtcgcacac gcaccgaggc aaaggagtcg cgcacagtct catcgaattt
16621 gcgaaaaagt gggcactaag cagacagctc cttggcatac gattagagac acaaacgaac
16681 aatgtacctg cctgcaattt gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg
16741 ttcacgtata aaactagacc tcaagtctcg aacgaaacag cgatgtactg gtactggttc
16801 tcgggagcac aggatgacgc ctaacaattc attcaagccg acaccgcttc gcggcgcggc
16861 ttaattcagg agttaaacat catgagggaa gcggtgatcg ccgaagtatc gactcaacta
16921 tcagaggtag ttggcgtcat cgagcgccat ctcgaaccga cgttgctggc cgtacatttg
16981 tacggctccg cagtggatgg cggcctgaag ccacacagtg atattgattt gctggttacg
17041 gtgaccgtaa ggcttgatga aacaacgcgg cgagctttga tcaacgacct tttggaaact
17101 tcggcttccc ctggagagag cgagattctc cgcgctgtag aagtcaccat tgttgtgcac
17161 gacgacatca ttccgtggcg ttatccagct aagcgcgaac tgcaatttgg agaatggcag
17221 cgcaatgaca ttcttgcagg tatcttcgag ccagccacga tcgacattga tctggctatc
17281 ttgctgacaa aagcaagaga acatagcgtt gccttggtag gtccagcggc ggaggaactc
17341 tttgatccgg ttcctgaaca ggatctattt gaggcgctaa atgaaacctt aacgctatgg
17401 aactcgccgc ccgactgggc tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt
17461 tggtacagcg cagtaaccgg caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg
17521 gagcgcctgc cggcccagta tcagcccgtc atacttgaag ctaggcaggc ttatcttgga
17581 caagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgttca ctacgtgaaa
17641 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc
17701 ttcgcggcgc ggcttaactc aagcgttaga gagctgggga agactatgcg cgatctgttg
17761 aaggtggttc taagcctcgt acttgcgatg gcatcggggc aggcacttgc tgacctgcca
17821 attgttttag tggatgaagc tcgtcttccc tatgactact ccccatccaa ctacgacatt
17881 tctccaagca actacgacaa ctccataagc aattacgaca atagtccatc aaattacgac
17941 aactctgaga gcaaatacga taatagttca tccaattacg acaatagtcg caacggaaat
18001 cgtaggctta tatatagcgc aaatgggtct cgcactttcg ccggctacta cgtcattgcc
18061 aacaatggga caacgaactt cttttccaca tctggcaaaa ggatgttcta caccccaaaa
18121 ggggggcgcg gcgtctatgg cggcaaagat gggagcttct gcggggcatt ggtcgtcata
18181 aatggccaat tttcgcttgc cctgacagat aacggcctga agatcatgta tctaagcaac
18241 tagcctgctc tctaataaaa tgttaggagc ttggctgcca tttttggggt gaggccgttc
18301 gcggccgagg ggcgcagccc ctggggggat gggaggcccg cgttagcggg ccgggagggt
18361 tcgagaaggg ggggcacccc ccttcggcgt gcgcggtcac gcgcacaggg cgcagccctg
18421 gttaaaaaca aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg
18481 gccgaaaaac gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc
18541 aaatgtcaat aggtgcgccc ctcatctgtc agcactctgc ccctcaagtg tcaaggatcg
18601 cgcccctcat ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc
18661 caggcttgtc cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg
18721 cgaggctggc cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc
18781 cgcgccgggt gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc
18841 caagttttcc gcgaggtatc cacaacgccg gcggccggcc gcggtgtctc gcacacggct
18901 tcgacggcgt ttctggcgcg tttgcagggc catagacggc cgccagccca gcggcgaggg
18961 caaccagccc ggtgagcgtc ggaaagggtc gaggaattcc gcctaaccgg ctcagttctg
19021 cgtagaaacc aacatgcaag ctccaccggg tgcaaagcgg cagcggcggc aggatatatt
19081 caattgtaaa tggcttcatg tccgggaaat ctacatggat cagcaatgag tatgatggtc
19141 ccatcgataa gcttggcaat tcccgatcta gtaacataga tgacaccgcg cgcgataatt
19201 tatcctagtt tgcgcgctat attttgtttt ctatcgcgta ttaaatgtat aattgcggga
19261 ctctaatcat aaaaacccat ctcataaata acgtcatgca ttacatgtta attattacat
19321 gcttaacgta attcaacaga aattatatga taatcatcgc aagaccggca acaggattca
19381 atcttaagaa actttattgc caaatgtttg aacgatcggg gatcgatccc ggtcggcatc
19441 tactctattc ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta
19501 cttctacaca gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga
19561 cagtcccggc tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat
19621 cgaaattgcc gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg
19681 cccggagccg cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct
19741 gctccataca agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat
19801 ccccgaacat cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga
19861 cattgttgga gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc
19921 aaagcatcag ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag
19981 tttgccagtg atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt
20041 attgaccgat tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg
20101 cagcgatcgc atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag
20161 gcaggtcttg caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc
20221 tgaatgcccc aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat
20281 aaacataacg atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac
20341 gccctcctac atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt
20401 cggagacgct gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag
20461 gctttttcat atcttattgc ccccctagag tcgagatctg gattgagagt gaatatgaga
20521 ctctaattgg ataccgaggg gaatttatgg aacgtcagtg gagcattttt gacaagaaat
20581 atttgctagc tgatagtgac cttaggcgac ttttgaacgc gcaataatgg tttctgacgt
20641 atgtgcttag ctcattaaac tccagaaacc cgcggctgag tggctccttc aacgttgcgg
20701 ttctgtcagt tccaaacgta aaacggcttg tcccgcgtca tcggcggggg tcataacgtg
20761 actcccttaa ttctccgctc atgatcttga tcccctgcgc catcagatcc ttggcggcaa
20821 gaaagccatc cagtttactt tgcagggctt cccaacctta ccagagggcg ccccagctgg
20881 caattccggt tcgcttgctg tccataaaac cgcccagtct agctatcgcc atgtaagccc
20941 actgcaagct acctgctttc tctttgcgct tgcgttttcc cttgtccaga tagcccagta
21001 gctgacattc atccggggtc agcaccgttt ctgcggactg gctttctacg tgttccgctt
21061 cctttagcag cccttgcgcc ctgagtgctt gcggcagcgt gaagcttgca tgcctgcagg
21121 tcgactctag acacggggtg gtttggttta aacaaaaaaa gcaccgactc ggtgccactt
21181 tttcaagttg ataacggact agccttattt taacttgcta tttctagctc taaaacctat
21241 atatacttac tacacacaat cactacttcg actctagctg tatataaact cagcttcgtt
21301 ttcttatcta agcgatgtgg gacttttgaa gattgttttc aacttaaatg ggcctatata
21361 agaaatacta ttgttctttc ccatataaat gggcctgctt ctcttctttc agattcccag
21421 gggccttttg aagattatct tcatatctta agaatgaaga tgttttattc aatcaaattc
21481 ttgaaggttc gatgcctaat cattctaatc ctgggacaaa ctatgaaaca agatacaaaa
21541 actccgaatg gaaagttaaa aagaagaaaa cgaaagctac ggttcaagaa aatgtaagct
21601 gataaacaaa aaaaaactgt atgaacgaag aagaagaaaa aaagctaaga agaaatgatg
21661 tattgtgcgg aaggcaagtc gagtttccgt tgttcaacga aagcttgttt aaaaaaagca
21721 ccgactcggt gccacttttt caagttgata acggactagc cttattttaa cttgctattt
21781 ctagctctaa aactgtaggc ttacgctata aatcaatcac tacttcgact ctagctgtat
21841 ataaactcag cttcgttttc ttatctaagc gatgtgggac ttttgaagat tgttttcaac
21901 ttaaatgggc ctatataaga aatactattg ttctttccca tataaatggg cctgcttctc
21961 ttctttcaga ttcccagggg ccttttgaag attatcttca tatcttaaga atgaagatgt
22021 tttattcaat caaattcttg aaggttcgat gcctaatcat tctaatcctg ggacaaacta
22081 tgaaacaaga tacaaaaact ccgaatggaa agttaaaaag aagaaaacga aagctacggt
22141 tcaagaaaat gtaagctgat aaacaaaaaa aaactgtatg aacgaagaag aagaaaaaaa
22201 gctaagaaga aatgatgtat tgtgcggaag gcaagtcgag tttccgttgt tcaacgaaag
22261 ctttcctaaa cgtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
22321 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
22381 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
22441 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
22501 ccaaatgttt gaacgatcgg ggaaattcga gctcaaactt acggccatgg cgtaggcggc
22561 cttttccgtt gtgatcttcg cggtctcctc cttgtttttg gactagtacc accaccgcta
22621 ccaccacctt cggttaccgt gaaggttttg gtagcgtcgt cgtaggtcca ttcaccgtca
22681 acaccgttgt cgttagcgta ctgtttgaaa actttttccg cggtagcagc gtcaacagct
22741 tcggtggtgg tttcaccttt cagggtttta ccgttcagga taagcttgta ctcttcggtc
22801 cgagatcctc ctcccgtacg gacctttctc ttcttctttg gagcccctcc gccaccgcca
22861 ctacctagca tatctagatc aaagtcatct agggcatcgg agcccaacat atctaaatcg
22921 aagtcgtcca atgcatctga gcctaacatg tctaggtcga agtcgtctaa cgcgtctgaa
22981 ccaagcatat ctaagtcaaa atcgtctaaa gcatcccctc cgccaccgcc cgtacgaccg
23041 gtccgacctc cacctttgta gagctcatcc atgccatgtg taatcccagc agcagttaca
23101 aactcaagaa ggaccatgtg gtcacgcttt tcgttgggat ctttcgaaag gacagattgt
23161 gtcgacaggt aatggttgtc tggtaaaagg acagggccat cgccaattgg agtattttgt
23221 tgataatggt ctgctagttg aacggaacca tcttcaacgt tgtggcgaat tttgaagtta
23281 gctttgattc cattcttttg tttgtctgcc gtgatgtata cattgtgtga gttaaagttg
23341 tactcgagtt tgtgtccaag aatgtttcca tcttctttaa aatcaatacc ctttaactcg
23401 atacgattaa caagggtatc accttcaaac ttgacttcag cacgcgtctt gtaggtcccg
23461 tcatctttga aagatatagt gcgttcctgt acataacctt cgggcatggc actcttgaaa
23521 aagtcatgcc gtttcatgtg atccggataa cgggaaaagc attgaacacc ataggtcaga
23581 gtagtgacaa gtgttggcca cggaacaggt agttttccag tagtgcaaat aaatttaagg
23641 gtgagttttc cgtttgtagc atcaccttca ccctctccac ggacagaaaa tttgtgccca
23701 ttaacatcac catctaattc aacaagaatt gggacaactc cagtgaaaag ttcttctcct
23761 ttgctaccgc ttccacctcc acctggatcc aagcttccgc cgcctcctga accaccaccg
23821 ccactacctc ctcccccaga acctccgcct ccaccagcgt aatctggaac atcgtatggg
23881 tagctgctca cggtcaccag ggtgccctgg ccccagtagt cgaacaggcc ggtcacgcag
23941 tagtacaggg cggtgtcgtc gctgcgcacc ttgctcatct gcaggtacac ggtgttcttg
24001 ccgttgtcct tgctgatgat gaagcggtcc ttcagggcgc tgttgtagtc ggtgatgccg
24061 tcgccccaga tcacgccgat ccactccagg ccgcggccgg gggcctggcg cacccagttc
24121 acgccgtagt cggtcaggct gaagccgctc acggcgcagc tcagcttcag gctgccgccg
24181 ggctgcacca ggccgccgcc gctctccagc agcttcacct cgctgccgcc gccgctgctg
24241 ccgccgccgc cgctgccgcc gccgccgctg ccgccgccgc cgcgcttcag ctccaccttg
24301 gtgccctggc cgaacaccca gtggttgctg taccacaggg cgcagaagta ggtggcgaag
24361 tcctcgggct gcaggctgct gatggtcagg gtggccttgt cgccgatcag gctgccgctg
24421 aagcggctgg gcacgccggg ggcgcggttg ttggtgccgc cgatcaggcc cttgaacagc
24481 ttgccgggct tctcctgcac ccagctggcg tagttgctgg tggtcacggc gccggtgctg
24541 ctgcggcagg tgatggtcac gcggtcgccc acgctggcgc tcaggctgct ggggctctgg
24601 gtcatcacga tgtcggggcc cataaaccct aggtgttaat cagaaaaact cagattaatc
24661 gacaaattcg atcgcacaaa ctagaaacta acaccagatc tagatagaaa tcacaaatcg
24721 aagagtaatt attcgacaaa actcaaatta tttgaacaaa tcggatgata tctatgaaac
24781 cctaatcgag aattaagatg atatctaacg atcaaaccca gaaaatcgtc ttcgatctaa
24841 gattaacaga atctaaacca aagaacatat acgaaattgg gatcgaacga aaacaaaatc
24901 gaagattttg agagaataag gaacacagaa atttaccttg atcacggtag agagaattga
24961 gagaaagttt ttaagatttt gagaaattga aatctgaatt gtgaagaaga agagctcttt
25021 gggtattgtt ttatagaaga agaagaagaa aagacgagga cgactaggtc acgagaaagc
25081 taaggcggtg aagcaatagc taataataaa atgacacgtg tattgagcgt tgtttacacg
25141 caaagttgtt tttggctaat tgccttattt ttaggttgag gaaaagtatt tgtgctttga
25201 gttgataaac acgactcgtg tgtgccggct gcaaccactt tgacgccgtt tattactgac
25261 tcgtcgacaa ccacaatttc taacggtcgt cataagatcc agccgttgag atttaacgat
25321 cgttacgatt tatatttttt tagcattatc gttttatttt ttaaatatac ggtggagctg
25381 aaaattggca ataattgaac cgtgggtccc actgcattga agcgtatttc gtattttcta
25441 gaattcttcg tgctttattt cttttccttt ttgttttttt ttgccattta tctaatgcaa
25501 gtgggcttat aaaatcagtg aatttcttgg aaaagtaact tctttatcgt ataacatatt
25561 gtgaaattat ccatttcttt taatttttta gtgttattgg atatttttgt atgattattg
25621 atttgcatag gataatgact tttgtatcaa gttggtgaac aagtctcgtt aaaaaaggca
25681 agtggtttgg tgactcgatt tattcttgtt atttaattca tatatcaatg gatcttattt
25741 ggggcctggt ccatatttaa cactcgtgtt cagtccaatg accaataata ttttttcatt
25801 aataacaatg taacaagaat gatacacaaa acattctttg aataagttcg ctatgaagaa
25861 gggaacttat ccggtcctag atcatcagtt catacaaacc tccatagagt tcaacatctt
25921 aaacaagaat atcctgatct gaagaatgtg gaggctttag tcccttggat acttgggagg
25981 ctgtggaaga acagaaacga gctggtgctt aaagggaggg aatttggaac caatgaggta
26041 ttagtaagga caca
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pBAtC
- 下一篇：pGST-PPARgamma
